Skip to main content
SpringerLink
Log in

Characterization of a 130,000-dalton glycoprotein isolated from pulmonary secretions of patients with alveolar proteinosis

  • Original Articles
  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

A new glycoprotein with an apparent molecular weight of 130,000 was isolated, purified, and partially characterized from the pulmonary secretions which accumulate so massively in the lungs of patients suffering from pulmonary alveolar proteinosis. The amino acid analysis of the glycoprotein showed the presence of relatively high amounts of glycine, glutamic acid, aspartic acid, leucine, and valine, and small amounts of hydroxyproline, but no hydroxylysine. It contains approximately 6% hexose, 3% sialic acid, and 4% glucosamine. The neutral sugars are galactose, mannose, and fucose. This alveolar glycoprotein cross-reacted with an antiserum prepared in rabbits against a larger glycoprotein (250,000 mol wt) isolated from the same source, suggesting that the larger alveolar glycoprotein may be the precursor of the smaller one.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Sahu, S., R. P. DiAgustine, andW. S. Lynn. 1976. Lipids found in pulmonary lavage of patients with alveolar proteinosis and in rabbit lung lamellar organelles.Am. Rev. Resp. Dis. 114:177–185.

    Google Scholar 

  2. Kylstra, J. A., D. C. Rousch, D. K. Hall, andA. Spock. 1971. Volume-controlled lung lavage in the treatment of asthma, bronchictasis and mucoviscidosis.Am. Rev. Resp. Dis. 103:651–665.

    Google Scholar 

  3. Sahu, S., andW. S. Lynn. 1979. Characterization of a high-molecular-weight glycoprotein isolated from the pulmonary secretions of patients with alveolar proteinosis.Biochem. J. 177:153–158.

    Google Scholar 

  4. Bhattacharyya, S. N., andW. S. Lynn. 1977. Studies on structural relationship between two glycoproteins isolated from alveoli of patients with alveolar proteinosis.Biochim. Biophys. Acta 494:150–161.

    Google Scholar 

  5. Bhattacharyya, S. N., S. Sahu, andW. S. Lynn. 1976. Structural studies on a glycoprotein isolated from alveoli of patients with alveolar proteinosis.Biochim. Biophys. Acta 427:91–106.

    Google Scholar 

  6. Sahu, S., andW. S. Lynn. 1977. Phospholipase A in pulmonary secretions of patients with alveolar proteinosis.Biochim. Biophys. Acta 487:354–360.

    Google Scholar 

  7. Sahu, S., andW. S. Lynn. 1977. Characterization of phospholipase A from pulmonary secretions of patients with alveolar proteinosis.Biochim. Biophys. Acta 489:307–317.

    Google Scholar 

  8. Sahu, S., andW. S. Lynn. 1978. Hyaluronic acid in the pulmonary secretions of patients with alveolar proteinosis.Inflammation 3:149–158.

    Google Scholar 

  9. Forstner, J. F., I. Jabbal, andG. G. Forstner. 1973. Goblet cell mucin of rat small intestine: chemical and physical characterization.Can. J. Biochem. 51:1154–1166.

    Google Scholar 

  10. Lowry, O. H., N. J. Rosenbrough, A. L. Farr, andR. J. Randall. 1951. Protein estimation with Folin phenol reagent.J. Biol. Chem. 193:265–275.

    Google Scholar 

  11. Dubois, M., G. A. Gilles, J. K. Hamilton, P. A. Rebers, andF. Smith. 1956. Colorimetric method for determination of sugars.Anal. Chem. 28:350–356.

    Google Scholar 

  12. Spiro, R. G. 1972. Study of the carbohydrates in glycoproteins.Methods Enzymol. 28:343.

    Google Scholar 

  13. Warren, L. 1959. The thiobarbituric acid assay of sialic acids.J. Biol. Chem. 234:1971–1975.

    Google Scholar 

  14. Weber, K., andM. Osborn. 1975. Proteins and sodium dodecyl sulfate: Molecular weight determination on polyacrylamide gels and related procedures.In The Proteins, Vol. 1. H. Neurath, and R. L. Hill, editors. Academic Press, New York. 179–223.

    Google Scholar 

  15. Glossmann, H. G., andD. M. Neville. 1971. Glycoproteins of cell surfaces.J. Biol. Chem. 246:6339–6349.

    Google Scholar 

  16. Weber, K., andM. Osborn. 1969. The reliability of molecular weight determinations by dodecyl sulfate polyacrylamide gel electrophoresis.J. Biol. Chem. 244:4406–4412.

    Google Scholar 

  17. Sear, C. H. J., M. E. Grant, andD. S. Jackson. 1977. Biosynthesis and release of glycoproteins by human skin fibroblasts in culture.Biochem. J. 168:91–103.

    Google Scholar 

  18. Anderson, L. C., C. G. Gahmberg, A. K. Kimura, andH. Wigzell. 1978. Activated human T lymphocytes display new surface glycoproteins.Proc. Natl. Acad. Sci. U.S.A. 75:3455–3458.

    Google Scholar 

  19. Carter, W. G., andS. Hakomori. 1977. Isolation and partial characterization of “galactoprotein a” and “galactoprotein b” from hamster embryo fibroblasts.Biochem. Biophys. Res. Commun. 76:299–308.

    Google Scholar 

  20. Daniels, J. R., andG. H. Chu. 1975. Basement membrane collagen of renal glomerulus.J. Biol. Chem. 250:3531–3537.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Biochemistry and Medicine, Duke University Medical Center, 27710, Durham, North Carolina

    Saura Sahu & William S. Lynn

Authors
  1. Saura Sahu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. William S. Lynn
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sahu, S., Lynn, W.S. Characterization of a 130,000-dalton glycoprotein isolated from pulmonary secretions of patients with alveolar proteinosis. Inflammation 3, 437–445 (1979). https://doi.org/10.1007/BF00913501

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00913501

Keywords

Search

Navigation