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Persistence of circulating endothelial microparticles in COPD despite smoking cessation
  1. Yael Strulovici-Barel1,
  2. Michelle R Staudt1,
  3. Anja Krause1,
  4. Cynthia Gordon1,
  5. Ann E Tilley1,
  6. Ben-Gary Harvey1,2,
  7. Robert J Kaner1,2,
  8. Charleen Hollmann1,
  9. Jason G Mezey1,3,
  10. Hans Bitter4,5,
  11. Sreekumar G Pillai4,6,
  12. Holly Hilton4,7,
  13. Gerhard Wolff4,8,
  14. Christopher S Stevenson4,
  15. Sudha Visvanathan4,9,
  16. Jay S Fine4,9,
  17. Ronald G Crystal1
  1. 1Department of Genetic Medicine, Weill Medical College of Cornell University, New York, New York
  2. 2Department of Pulmonary and Critical Care Medicine, Weill Medical College of Cornell University, New York, New York
  3. 3Department of Biological Statistics and Computational Biology, Cornell University, Ithaca, New York, USA
  4. 4Hoffmann-La Roche, Nutley, New Jersey, USA
  5. 5Novartis
  6. 6Eli Lilly and Company
  7. 7PPD Labs
  8. 8Achillion Pharmaceuticals, Inc.
  9. 9Boehringer Ingelheim Pharmaceuticals
  1. Correspondence to Dr Ronald G Crystal, Department of Genetic Medicine, Weill Cornell Medical College, 1300 York Avenue, Box 164, New York, NY 10065, USA; geneticmedicine{at}med.cornell.edu

Abstract

Introduction Increasing evidence links COPD pathogenesis with pulmonary capillary apoptosis. We previously demonstrated that plasma levels of circulating microparticles released from endothelial cells (EMPs) due to apoptosis are elevated in smokers with normal spirometry but low diffusion capacity, that is, with early evidence of lung destruction. We hypothesised that pulmonary capillary apoptosis persists with the development of COPD and assessed its reversibility in healthy smokers and COPD smokers following smoking cessation.

Methods Pulmonary function and high-resolution CT (HRCT) were assessed in 28 non-smokers, 61 healthy smokers and 49 COPD smokers; 17 healthy smokers and 18 COPD smokers quit smoking for 12 months following the baseline visit. Total EMP (CD42bCD31+), pulmonary capillary EMP (CD42bCD31+ACE+) and apoptotic EMP (CD42bCD62E+/CD42bCD31+) levels were quantified by flow cytometry.

Results Compared with non-smokers, healthy smokers and COPD smokers had elevated levels of circulating EMPs due to active pulmonary capillary endothelial apoptosis. Levels remained elevated over 12 months in healthy smokers and COPD smokers who continued smoking, but returned to non-smoker levels in healthy smokers who quit. In contrast, levels remained significantly abnormal in COPD smokers who quit.

Conclusions Pulmonary capillary apoptosis is reversible in healthy smokers who quit, but continues to play a role in COPD pathogenesis in smokers who progressed to airflow obstruction despite smoking cessation.

Trial registration number NCT00974064; NCT01776398.

  • Smoking cessation
  • COPD ÀÜ Mechanisms
  • Tobacco and the lung

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