Fever, splenomegaly and lymphopenia in sarcoidosis
- 1Department of Diseases of the Thorax, Pulmonology Unit, Pierantoni-Morgagni Hospital, Forlì, Italy
- 2Department of Pathology, Pierantoni-Morgagni Hospital, Forlì, Italy
- Correspondence to Dr Venerino Poletti, Pulmonology, Department of Thoracic Diseases, GB Pierantoni—L Morgagni Hospital, via C. Forlanini 34, 47100 Forlì, Italy;
Contributors All authors have made important contributions in the discussion of the clinical case and drafting the article.
- Infection control
- opportunist lung infections
- COPD Pathology
- drug induced lung disease
- interstitial fibrosis
- lung cancer
- lung cancer chemotherapy
- pleural disease
- alveolar cell cancer
- anca related vasculitides
- eosinophil biology
- lymphocyte biology
- macrophage biology
- respiratory infection
A 42-year-old woman was referred to our department with a 5 months history of intermittent fever and fatigue. Her past medical history included a consolidated diagnosis of sarcoidosis (obtained 7 years before with a sub-carinal lymph node biopsy) and in the previous years she had been treated with steroids, hydroxychloroquine, methotrexate and azathioprine with persistent and progressive enlargement of mediastino-hilar adenopathies and bilateral nodular infiltrates. Her symptoms progressed despite a course of antibiotics prescribed for presumed community-acquired pneumonia and a course of corticosteroids. The patient lived in Italy and there was no history of travels or other additional risk factors for infections.
At the time of admission, the patient had a temperature of 38.5°C and the physical examination was only notable for hepatomegaly and splenomegaly. A white blood cell count showed lymphopenia (0.39×10^9/l, CD4+ 0.22×10^9/l) and anaemia (Hb 10.4 g/dl, MCV 78.7 fl).
CT images of the thorax revealed bilateral nodules of variable sizes predominantly in the upper lung fields and a positron emission tomography showed several areas of increased metabolism in the liver, spleen, lymph nodes (above and under the diaphragm), D3 vertebra and bilateral nodular infiltrates (figure 1).
Fibre-optic bronchoscopy was normal and all investigations for infections (blood, bronchoalveolar lavage, urine and serological tests) were negative. ECG was normal.
A bone marrow biopsy was performed: the perivascular region was characterised by a concrete number of polyclonal mature plasma cells (for κ and λ light chains of immunoglobulins) and a diffuse interstitial infiltration of foamy macrophages with intracellular inclusions was noted as showed in figure 2.
From the question on page above
The pathological analysis of the bone marrow revealed diffuse interstitial infiltration of foamy macrophages containing Leishmania protozoa. The conclusive diagnosis was visceral leishmaniosis in immunocompromised patient. The patient was treated with liposomial amphotericin (3.5 mg/kg for 5 days and then with a maintenance dose); treatment was well tolerated and resulted in immediate regression of the fever and improvement in general state of health; C-reactive protein decreased from 87 mg/l to 24 mg/l and lymphocytes increased from 0.39 mg/l to 0.95 mg/l.
Fever, splenomegaly and lymphopenia may arise from a large range of infectious, haematological or systemic diseases and therefore represent a difficult diagnostic challenge.1 In this case, the previous history of sarcoidosis was an apparent clue; however, we know from literature that sarcoidosis and lymphoma, mainly non-Hodgkin's lymphoma, may occur together, with sarcoidosis usually preceding lymphoma2 and the coexistence of sarcoidosis and opportunistic infection, even in the absence of any immunosuppressive therapy, has previously been documented.3 A possible infectious cause of fever, splenomegaly and lymphopenia is visceral leishmaniasis (VL, kala-azar), a systemic infection of the reticuloendothelial system caused by protozoa of the genus Leishmania. The definitive diagnosis of kala-azar requires demonstration or isolation of parasites from samples collected by invasive organ aspiration.4 VL is endemic in areas bordering the Mediterranean Sea; even though leishmaniasis is seen relatively infrequently in connection with sarcoidosis, our case presentation demonstrates that VL must be taken into consideration in the differential diagnosis of febrile splenomegaly in patients living in areas endemic for such protozoa.5
Competing interests None.
Patient consent Obtained.
Ethics approval Ethics approval was provided by Area Vasta Romagna Review Board.
Provenance and peer review Not commissioned; externally peer reviewed.
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-commercial License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited, the use is non commercial and is otherwise in compliance with the license. See: http://creativecommons.org/licenses/by-nc/2.0/ and http://creativecommons.org/licenses/by-nc/2.0/legalcode.