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Bronchial epithelial cells cultured from clinically stable lung allograft patients promote the development of macrophages from monocytes rather than dendritic cells.
  1. Christopher Ward (chris.ward{at}ncl.ac.uk)
  1. University of Newcastle upon Tyne, United Kingdom
    1. Katrien Eger
    1. University of Leiden, Netherlands
      1. Julie Diboll
      1. University of Newcastle upon Tyne, United Kingdom
        1. Debbie Jones
        1. University of Newcastle upon Tyne, United Kingdom
          1. Muzlifah A Haniffa
          1. University of Newcastle upon Tyne, United Kingdom
            1. Andrew Fisher
            1. University of Newcastle upon Tyne, United Kingdom
              1. Malcolm Brodlie
              1. University of Newcastle upon Tyne, United Kingdom
                1. James L Lordan
                1. University of Newcastle upon Tyne, United Kingdom
                  1. Paul A Corris
                  1. University of Newcastle upon Tyne, United Kingdom
                    1. Catharien M U Hilkens
                    1. University of Newcastle upon Tyne, United Kingdom

                      Abstract

                      Introduction: Chronic allograft failure occurs as a result of alloimmune and non-allo-immune injury. Dendritic cells (DC) are thought to be crucial in regulating (allo)immune airway damage and interactions with epithelial cells are likely. Studies in human lung transplantation are limited however, and the available literature on DC inconsistent. Our study focused on the ex-vivo influence of primary bronchial epithelial cells derived from lung allografts on DC differentiation.

                      Methods: Epithelial cell conditioned media (ECCM) were added to monocytes differentiating into DC under the influence of Interleukin-4 and Granulocyte Macrophage-Colony Stimulating Factor. The resultant cells were compared to DC cultured without ECCM and to monocyte-derived macrophages. Expression of typical DC (e.g. CD1a) and macrophage (e.g. CD14) markers was assessed by flow cytometry. Phenotypical assessments were complimented by functional studies of mannose receptor-mediated phagocytosis (FITC dextran uptake) and antigen-presenting capability (Mixed Lymphocyte Reactions).

                      Results: Cells exposed to ECCM expressed statistically significantly, lower levels of CD1a than unexposed DC. CD14 expression was increased, as was phagocytic function. ECCM-cultured cells also expressed lower levels of T-cell co-stimulatory molecules, secreted an anti-inflammatory cytokine profile and had significantly reduced antigen-presenting capability.

                      Conclusion: Using phenotypic and functional approaches our study has shown that ECCM from lung allografts drives the production of macrophage-like cells from monocytes, rather than DC. Our data suggest that epithelial cells may restrain airway DC and potential alloimmunity. It remains unclear whether the observed effect is specifically seen in lung transplant recipients or might be a general property of bronchial epithelial cells. It is possible that this may reflect a homeostatic inter-relationship between airway epithelial and DC populations, relevant both to lung allografts and the lung more generally.

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