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Soluble Endostatin is a novel inhibitor of epithelial repair in Idiopathic Pulmonary Fibrosis
  1. Alex G Richter (alex.richter{at}blueyonder.co.uk)
  1. Birmingham University, United Kingdom
    1. Scott McKeown
    1. Queen's University Belfast, United Kingdom
      1. Sridhar Rathinam
      1. Heartlands Hospital, United Kingdom
        1. Lorraine Harper
        1. University of Birmingham, United Kingdom
          1. Pala B Rajesh
          1. Heartlands Hospital, United Kingdom
            1. Danny F McAuley (d.f.mcauley{at}qub.ac.uk)
            1. Queen's University belfast, United Kingdom
              1. Ritva Heljasvaara
              1. Universtiy of Oulu, Finland
                1. David R Thickett (d.thickett{at}bham.ac.uk)
                1. University of Birmingham, United Kingdom

                  Abstract

                  Aberrant angiogenesis and defective epithelial repair are key features of Idiopathic pulmonary fibrosis (IPF). Endostatin is an anti-angiogenic peptide with known effects on endothelial cells. This study aimed to establish the levels of endostatin in the bronchoalveolar lavage fluid (BALF) in IPF and to investigate its actions on distal lung epithelial cells (DLEC) and primary type II cells.

                  Methods: 20 patients with IPF and 10 controls underwent BAL. Endostatin was measured by ELISA. BALF cytokines and Matrix Metalloproteinase (MMP) -3 were measured by Luminex array. Primary DLEC monolayers were wounded and treated with endostatin. Apoptosis and cell viability were assessed.

                  Results: Endostatin was elevated in the BALF and plasma of patients with IPF compared with normal controls. There was a negative correlation between endostatin, forced vital capacity and gas transfer. Endostatin correlated with a number of pro-inflammatory cytokines and MMP-3. Physiological endostatin doses inhibited DLEC wound repair by 44% in an effect that was partially FasL and caspase dependent. Endostatin increased apoptosis rates by 8% and reduced their viability by 34%. Similar effects of endostatin were seen in primary type II cells in terms of inhibition of wound repair and proliferation.

                  Conclusions: Elevated BALF endostatin levels correlated with a number of elevated cytokines, MMP-3 and lung function in IPF. Endostatin is a novel inhibitor of DLEC wound repair, inducing apoptosis and reducing cell viability in a FasL and caspase dependent manner. Endostatin may play a role in aberrant epithelial repair in IPF.

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