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Pneumocystis Colonisation Is Common Among Hospitalized HIV-infected Patients with Non-Pneumocystis Pneumonia
  1. J. Lucian Davis (lucian.davis{at}ucsf.edu)
  1. University of California, San Francisco, United States
    1. David A Welsh (dwelsh{at}lsuhsc.edu)
    1. Louisiana State University Health Sciences Center, United States
      1. C. Ben Beard (cbb0{at}cdc.gov)
      1. Centers for Disease Control and Prevention, United States
        1. Jefffrey L Jones (jlj1{at}cdc.gov)
        1. Centers for Disease Control and Prevention, United States
          1. Gena G Lawrence (geg7{at}cdc.gov)
          1. Centers for Disease Control and Prevention, United States
            1. Melissa R Fox (mif0{at}cdc.gov)
            1. Centers for Disease Control and Prevention, United States
              1. Kristina Crothers (kristina.crothers{at}yale.edu)
              1. Yale University, United States
                1. Alison Morris (morrisa{at}upmc.edu)
                1. University of Pittsburgh, United States
                  1. Denise Charbonnet (mchar1{at}lsuhsc.edu)
                  1. Louisiana State University Health Sciences Center, United States
                    1. Alexandra Swartzman (sasha.swartzman{at}gmail.com)
                    1. University of Oregon Health Sciences Center, United States
                      1. Laurence Huang (lhuang{at}php.ucsf.edu)
                      1. University of California, San Francisco, United States

                        Abstract

                        Background: When Pneumocystis DNA is recovered from respiratory specimens of patients without Pneumocystis pneumonia (PCP), patients are said to be colonised with Pneumocystis, although the significance of this state is unknown. Understanding risk factors for and outcomes of colonisation may provide insights into the life cycle and transmission dynamics of Pneumocystis jirovecii.

                        Methods: We performed a cross-sectional study of the prevalence and clinical predictors of Pneumocystis colonisation in 172 HIV-infected, PCP-negative inpatients undergoing diagnostic evaluation of 183 episodes of pneumonia at either the Medical Center of Louisiana at New Orleans between 2003 and 2005 or San Francisco General Hospital between 2000 and 2005. DNA was extracted from sputum and bronchoalveolar lavage specimens and amplified using a nested polymerase-chain-reaction assay at the mitochondrial large-subunit (18S) ribosomal-RNA locus. Colonisation was deemed present if Pneumocystis DNA was identified by both gel electrophoresis and direct DNA sequencing.

                        Results: 68% (117/172) of all patients were colonised with Pneumocystis. No strong associations with colonisation were identified for any demographic factors. Among clinical factors, having a CD4+ T-cell count less than or equal to 50 cells/uL (unadjusted Odds Ratio (OR) 2.4, 95% Confidence Interval (CI) 1.09 - 5.48, p=0.031) and using PCP prophylaxis (unadjusted OR 0.55, 95% CI 0.29 - 1.07, p=0.077) were associated with Pneumocystis colonisation, although the latter association may have been due to chance. After adjustment for CD4+ T-cell count, use of PCP prophylaxis was associated with a decreased odds of colonisation (adjusted OR 0.45, 95% CI: 0.21 - 0.98, p=0.045). Eleven patients who were colonised were subsequently readmitted for evaluation of a second episode of pneumonia; three were found to be colonised again, but none had PCP.

                        Conclusions: The majority of hospitalized HIV-infected patients with non-PCP pneumonia are colonised with Pneumocystis. Failure to use co-trimoxazole prophylaxis and severe immunosuppression are associated with an increase in the odds of colonisation. Pneumocystis colonisation among hospitalized patients does not commonly lead to PCP.

                        • Bronchoalveolar lavage
                        • Colonization
                        • HIV/AIDS
                        • Pneumocystis jirovecii
                        • Pneumonia

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