Article Text

other Versions

PDF

Increased alveolar nitric oxide concentration, and high levels of leukotriene B4 and 8-isoprostane in exhaled breath condensate in patients with asbestosis
  1. Hannele Lehtonen
  1. The Immunopharmacology Research Group, University of Tampere and Tampere University Hospital, Finland
    1. Panu Oksa
    1. Finnish Institute of Occupational Health, Finland
      1. Lauri Lehtimäki
      1. The Immunopharmacology Research Group, University of Tampere and Tampere University Hospital, Finland
        1. Anna Sepponen
        1. The Immunopharmacology Research Group, University of Tampere and Tampere University Hospital, Finland
          1. Riina Nieminen
          1. The Immunopharmacology Research Group, University of Tampere and Tampere University Hospital, Finland
            1. Hannu Kankaanranta
            1. The Immunopharmacology Research Group, University of Tampere and Tampere University Hospital, Finland
              1. Seppo Saarelainen
              1. Department of Respiratory Medicine, Tampere University Hospital, Finland
                1. Ritva Järvenpää
                1. Department of Radiology, Tampere University Hospital, Finland
                  1. Jukka Uitti
                  1. Finnish Institute of Occupational Health, Finland
                    1. Eeva Moilanen (eeva.moilanen{at}uta.fi)
                    1. The Immunopharmacology Research Group, University of Tampere and Tampere University Hospital, Finland

                      Abstract

                      Background: Inhaled asbestos fibres can cause inflammation and fibrosis in the lungs, called asbestosis. However, there are no non-invasive means to assess and follow the severity of the inflammation. Exhaled nitric oxide (NO) measured at multiple exhalation flow rates can be used to assess alveolar NO concentration and bronchial NO flux which reflect inflammation in lung parenchyma and airways, respectively. The aim of the present study was to investigate if exhaled NO or markers in exhaled breath condensate could be used to assess inflammation in asbestosis.

                      Methods: Exhaled NO and inflammatory markers (LTB4 and 8-isoprostane) in exhaled breath condensate were measured in fifteen non-smoking patients with asbestosis and in fifteen healthy controls. Exhaled NO concentrations were measured at four constant exhalation flow rates (50, 100, 200 and 300 ml/s), and alveolar NO concentration and bronchial NO flux were calculated according to the linear model of pulmonary NO dynamics.

                      Results: Alveolar NO concentration was significantly higher in patients with asbestosis (3.2 ± 0.4 ppb; mean ± SEM) than in controls (2.0 ± 0.2 ppb, p=0.008). There was no difference in bronchial NO flux (0.9 ± 0.1 vs 0.9 ± 0.1 nl/s, p=0.93) or NO concentration measured at ATS standard flow rate of 50 ml/s (20.0 ± 2.0 ppb vs 19.7 ± 1.8 ppb, p=0.89). Patients with asbestosis had elevated levels of leukotriene B4 (39.5 ± 6.0 pg/ml vs.15.4 ± 2.9 pg/ml, p= 0.002) and 8-isoprostane (33.5 ± 9.6 pg/ml vs.11.9 ± 2.8 pg/ml, p= 0.048) in exhaled breath condensate. Patients with asbestosis had elevated serum levels of CR (2.3 ± 0.3 vs. 1.1. ± 0.2 ug/ml, p=0.003), IL-6 (3.5 ± 0.5 vs. 1.7 ± 0.4 pg/ml, p=0.007), and MPO (356 ± 48 vs. 240 ± 20 ng/ml, p=0.034) as compared to healthy controls.

                      Conclusions: Patients with asbestosis had increased alveolar NO concentration and high levels of LTB4 and 8 isoprostane in exhaled breath. Measurement of exhaled NO at multiple exhalation flow rates and analysis of inflammatory markers in breath condensate are promising non-invasive means to assess inflammation in patients with asbestosis.

                      • alveolar NO concentration
                      • asbestosis
                      • bronchial NO flux
                      • exhaled NO
                      • exhaled breath condensate

                      Statistics from Altmetric.com

                      Request permissions

                      If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.