Introduction MicroRNAs (miRNAs) are small non-coding RNAs that function as endogenous gene regulators. They may initiate a process called epithelial-mesenchymal transition (EMT) that leads to aberrant extracellular matrix remodelling and is implicated in a number of airway diseases. Dysregulation of miRNAs has been indicated in chronic lung disorders, the third most common cause of mortality in adults.
Materials and methods NanoString was used to assay the differential expression of miRNAs at 1, 4 and 24 hrs following TGF-β1 treatment of BEAS-2B cells (immortalised primary bronchial epithelial cells) and control. QRT-PCR validated the expression profile of miR-200b. BEAS-2B and PBECs (primary bronchial epithelial cells) were transfected with miR-200b mimics to study expression of EMT markers at mRNA and protein level. MiRNA targets were identified and validated using multiple computational tools and qRT-PCR respectively.
Results nCounter assay allowed identification of novel miRNAs including miR-200 family. MiR-200b mimic transfection (24 hrs) followed by TGF-β1 treatment (48 hrs) demonstrated a significant increase in E-Cadherin (p ≤ 0.05, p ≤ 0.001) and a significant decrease in Fibronectin (p ≤ 0.001, p ≤ 0.01) in BEAS-2B cells and PBECs. Protein studies suggested a similar trend in both the cells. The most prominent targets of miR-200b identified were RHOA, SMURF2, ZNF532 and ZEB2. A significant decrease was observed in ZNF532 (p ≤ 0.01) and ZEB2 (p ≤ 0.001) in miR-200b transfected and TGF-β1 treated BEAS-2B cells (n = 3). Differential expression of mRNA targets was observed in two sets of patient derived PBECs.
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