Background The bronchial epithelium and underlying reticular basement membrane (RBM) have a close spatial and functional inter-relationship and are considered an epithelial–mesenchymal trophic unit (EMTU). An understanding of RBM development is critical to understanding the extent and time of appearance of its abnormal thickening that is characteristic of asthma.
Methods RBM thickness and epithelial height were determined in histological sections of cartilaginous bronchi obtained postmortem from 47 preterm babies and infants (median age 40 weeks gestation (22 weeks gestation–8 months)), 40 children (2 years (1 month–17 years)) and 23 adults (44 (17–90) years) who had died from non-respiratory causes, and had no history of asthma.
Results The RBM was visible by light microscopy at 30 weeks gestation. RBM thickness increased in successive age groups in childhood; in infants (r=0.63, p<0.001) and in children between 1 month and 17 years (r=0.82, p<0.001). After 18 years, RBM thickness decreased with increasing age (r=−0.42, p<0.05). Epithelial height showed a similar relationship with age, a positive relationship from preterm to 17 years (r=0.50, p<0.001) and a negative relationship in adulthood (r=−0.84, p<0.0001). There was a direct relationship between epithelial height and RBM thickness (r=0.6, p<0.001).
Conclusions The RBM in these subjects was microscopically identifiable by 30 weeks gestation. It thickened during childhood and adolescence. In adults, there was either no relationship with age, or a slow reduction in thickness in older age. Developmental changes of RBM thickness were accompanied by similar changes in epithelial height, supporting the close relationship between RBM and epithelium within the EMTU.
- Epithelial mesenchymal trophic unit
- reticular basement membrane
- airway epithelium
- paediatric lung disaese
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- Epithelial mesenchymal trophic unit
- reticular basement membrane
- airway epithelium
- paediatric lung disaese
The bronchial epithelial basement membrane is an acellular matrix composed of two structurally distinct layers, the basal lamina and the lamina reticularis or reticular basement membrane (RBM). The RBM is not present in healthy airways of all species. It is not present in rodents, but is present in cats, pigs, sheep1 and non-human primates.2 Its function and the reason for its presence in healthy humans remain unclear. Abnormal thickening of the RBM is a feature of airway remodelling seen in several inflammatory airway diseases and is especially characteristic of asthma. Biopsy studies in adults,3 children with severe4 and mild to moderate asthma5 and preschool children with severe wheezing6 have each reported increased RBM thickening as compared with non-asthma controls. Importantly, it has been proposed that direct interaction between the epithelium and underlying RBM is critical in the pathogenesis of asthma, and that they function as a single unit, the epithelial–mesenchymal trophic unit (EMTU).7 Although an increase in epithelial height in the first 8 months of life has been reported,8 subsequent changes in epithelial height with age are unknown. The relationship between RBM thickness and epithelial height during development of the EMTU has also not been investigated.
There are presently no data in humans on the timing of the first appearance and development of the bronchial epithelial RBM. Studies in monkeys have shown that the RBM develops postnatally, and is visible by light microscopy at 1 month postpartum, thickens further by 3 months and achieves final adult thickness by 6 months of age.9 Our current knowledge of normal RBM thickness in humans comes from endobronchial biopsy studies that have compared RBM thickness in patients with inflammatory airway conditions with controls. Ethical limitations mean only adult studies can include healthy volunteers as controls, and the range of reported normal values for RBM thickness in adult endobronchial biopsies is between 3.2 μm10 and 8.4 μm.11 In children, as endobronchial biopsy cannot be justified in healthy subjects, most studies have used surrogate controls, including children with isolated upper airway symptoms (stridor),6 and those with recurrent pneumonia or haemoptysis.4 To overcome the difficulty in obtaining tissue from healthy children, we used postmortem tissue from non-respiratory deaths which had been collected for clinical reasons. Importantly, RBM thickness measured in cartilaginous airways in autopsy tissue from adults with and without asthma has been shown to be similar to that measured in endobronchial biopsies taken from central airways.12 We hypothesised that the RBM is first visible at birth, thickens normally during the first few years of life and subsequently plateaus during school age, having by then reached its maximum normal thickness. We also reasoned that due to the close inter-relationship proposed between the RBM and overlying epithelium, changes in RBM thickness might be reflected by alterations in epithelial height.
Autopsy lung tissue was obtained from 47 fetuses and infants from London, UK (aged 22 weeks gestation to 8 months old) who were stillborn or had died suddenly without specific cause, 40 children from Sydney, Australia (aged 1 month to 17 years) and 23 adults from Perth, Australia (aged 17–90 years). All had undergone a postmortem examination for clinical reasons to establish the cause of death. In no case did the postmortem report reveal a primary lung abnormality or evidence of a history or pathological abnormalities of asthma or chronic obstructive pulmonary disease (COPD). Approval to use the tissue for the purpose of research had been obtained from the local ethics committees, and the laws on importation and research testing of human tissue obtained at autopsy had been complied with.
Tissue processing and staining
Lungs were collected and processed for histology as part of the postmortem study. Lungs from London and Sydney were fixed in 10% formalin; those from Perth were fixed in 10% paraformaldehyde. Tissue from London and Perth was fixed in inflation while that from Sydney was fixed by immersion. To exclude differences in RBM thickness resulting from fixation techniques, measurements from the three centres have been plotted separately. Tissue was stored in fixative for up to 6 months and processed through to wax embedding. 5 μm thick sections were cut and stained with H&E and used to quantify RBM thickness and epithelial height, and to measure internal airway perimeter.13
RBM measurement in whole airways
RBM thickness in tissue from London and Perth was measured by SS, while tissue from Sydney was measured by LT. Both observers were unaware of the subjects' clinical details. Interobserver agreement for measurements made in infants and children was assessed by a Bland–Altman plot of measurements from four airways performed by both observers (figure E1 online). RBM measurement was based on a previously validated method used in adult14 and paediatric4 endobronchial biopsies, and a previously described method from a study of whole airways in postmortem tissue.12 In order to limit the influence of airway size per se and to reflect the proximal airways accessed in endobronchial biopsy studies, all airways measured were intrapulmonary and cartilaginous, and those of approximately the third generation were measured when available. Sections were selected if the following additional criteria were satisfied: (1) airways had identifiable epithelium (as epithelium was stripped in some airways) and (2) airways were cut in a plane transverse to their length. Colour images of airways that satisfied these criteria were obtained with a digital camera (Zeiss Axiocam, Thame, UK). RBM thickness was measured using computer-aided image analysis (Openlab v3.02 software, Improvision, Coventry, UK). The internal airway perimeter was measured at a magnification of ×50 (figure 1A). Subsequently, four images (including epithelium, RBM and submucosal region) were taken at a magnification of ×400 from a random starting point and then at 90° intervals to allow measurement of RBM thickness and epithelial height. If the RBM could not be recognised anywhere along the airway, this was noted as a thickness of 0 μm. A line was drawn as a tangent to the outer (submucosal) edge of the RBM. RBM thickness was defined, measured and recorded along a line drawn towards the airway lumen, perpendicular to the tangent (figure 1B). A total of 40 measurements, 20 μm apart, were obtained from each airway, and the geometric mean was used to represent RBM thickness for that airway. If the RBM could be identified, but was too thin to be measured by the image analysis software, a score of 0.01 μm was recorded, and included in the final averaging of measurements. Between one and four airways were measured for each subject. The mean value from all airways was used for each subject when more than one airway was measured. The within-subject, between-airway coefficient of variation of RBM thickness in subjects with ≥3 analysable airways ranged between 5% and 23%.
Measurement of epithelial height
Bronchial epithelial height was measured using computer-aided image analysis at a magnification of ×400. Only intact epithelium, attached to the basement membrane along at least 0.5 mm length of the airway, was included. Epithelial height was measured from the luminal edge of the RBM to the luminal surface of a single cell, excluding cilia. The mean height of at least 10 cells from four different regions of the airway was taken to represent epithelial height for that airway.
Determination of airway size
Airway size was determined by assessment of its inner circumference, measured at magnification ×50 along the epithelial luminal edge (henceforth referred to as airway perimeter).
Linear relationships were assessed using Spearman rank correlation, with p<0.05 considered as statistically significant. Intraobserver repeatability of measurements was assessed by calculating the coefficient of variation (%) of measuring four airways on three different occasions. This ranged from 5.4% to 8.5% for SS and from 2.7% to 8.1% for LT. Data were analysed using SPSS version 11.5.
The median age at death for all fetuses and infants from London was 40 weeks gestation (range 22 weeks gestation–8 months), that for all children from Sydney was 2.3 years (range 1 month–17 years) and that for adults from Perth was 44 (range 18–90) years. Table 1 summarises the demographics of the subjects in the different age groups. Table 2 shows the cause of death for all subjects.
Relationship between RBM thickness and age
The RBM was not visible by light microscopy in 4 of the 27 preterm infants. It was only visible in one infant under 30 weeks gestation, but after this time it was visible in all preterm and post-term subjects. The association of RBM thickness and age is shown in figure 2A–C. When only preterm infants were assessed, RBM thickness was positively associated with age until term (r=0.6, p<0.001). From birth to age 8 months (London tissue), there was an increase in RBM thickness with age (r=0.63, p<0.001) (figure 2A). There was also a positive correlation between RBM thickness and age from infancy to age 17 years (Sydney tissue) (r=0.82, p<0.001) (figure 2B). Subsequently, from 18 years onwards (Perth tissue), there was a weak negative association between RBM thickness and age (Spearman r=−0.42, p<0.05) (figure 2C). When only tissue from Sydney was considered, the variance of RBM thickness in children ≥6 years old was significantly higher than in the younger group (variance ratio (F-test): 3.08, p<0.001), suggesting that the rate of increase was greater in those under 6 years than in those over 6 years old.
Relationships between epithelial height, RBM thickness and age
Epithelial height could be measured in 43/47 fetuses and infants (London tissue), 10/40 children (Sydney tissue) and 18/23 adults (Perth tissue). For airways in which epithelial height could be measured, there was a direct relationship between RBM thickness and epithelial height (Spearman r=0.6, p<0.001) (figure 3A). The relationships between epithelial height and age, and RBM thickness and age were similar (figure 3B–D). A positive relationship was apparent between age and epithelial height until 17 years (Spearman r=0.5, p<0.01) and a negative relationship between age and epithelial height after 18 years (Spearman r=−0.84, p<0.001).
Relationship between RBM thickness and airway size
Airways were chosen to reflect proximal airway generations from which endobronchial biopsies are taken. There was a positive relationship between internal airway perimeter and RBM thickness in Sydney children (Spearman r=0.576, p=0.001) (figure 4B). These specimens were fixed in immersion. There was no relationship between internal airway perimeter and RBM thickness in the infants (from London) or adults (from Perth) (figure 4A,C). Specimens from both of these centres were fixed in inflation. In order to determine whether the relationship between RBM thickness and age held true for the Sydney children, or whether the result might be confounded by the possibility of larger airways in older children, we determined the relationship between age and airway size. There was no significant relationship between age and airway size in any of the groups measured (figures 5A,B), thus supporting a true relationship between age and RBM thickness.
Relationship between gender, height and RBM thickness
RBM thickness was similar in males and females of similar ages (figure E2, online). There was a positive relationship between RBM thickness and subject height in the infants (Spearman r=0.54, p<0.001) and children (Spearman r=0.84, p<0.001), but not in the adults (Spearman r=0.37, p=0.08) (figure E3, online). There was no relationship between subject height and airway size in any of the age groups.
This is the first description of the time of first appearance and subsequent quantitative development of the RBM in humans without known respiratory disease. We have shown in our group of selected individuals that the RBM is difficult to discern by light microscopy before 30 weeks gestation, whereas between 30 weeks gestation and term, the RBM is visible in all of the cases studied. RBM thickness increases with age until term, subsequently increases rapidly until about 6 years of age and then continues to increase, but at a slower rate, until about 17 years. However, the large variability in RBM thickness between individuals after school age prevents the clear delineation of an age when its increase plateaus. In adulthood, RBM thickness appears to decrease gradually with age. We show for the first time a strong relationship between RBM thickness and epithelial height, and the change in epithelial height with age reflects the change in RBM thickness with age. These data support the proposed close interaction of the epithelium with its underlying RBM and their association as an EMTU.15
The first detection of the RBM by light microscopy ∼10 weeks before term (with one subject having a visible RBM at 24 weeks gestation) is consistent with a previous report of it being visible only by electron microscopy after ∼24 weeks gestation.16 It also highlights a key difference in RBM development between humans and primates, since in the latter it develops postnatally.2 The slope of the linear relationship between age and RBM thickness is steep from 30 weeks gestation up to 6 years of age (figure 2A,B), indicating a relatively rapid rate of increasing RBM thickness during this period. This is consistent with the rate of total lung growth which is fastest during the first 2 years of life, due primarily to alveolar multiplication.17 Alveoli also first appear in utero at ∼30 weeks gestation.18 Alveolar formation/maturation is controlled by interaction with the subepithelial elastin–collagen matrix, which acts as an anchoring fishnet through which alveoli protrude (ie, the ‘fishnet hypothesis’).19 The similar timing of onset of such a maturation process in the lung parenchyma and the appearance and rapid increase of bronchial RBM thickness suggests that both may be influenced by molecular changes in their adjacent lung connective tissue matrices.
We were unable to define clearly an age when maximal RBM thickness is reached. From our cross-sectional data it seems that the RBM increases in thickness, albeit at a slower rate after 6 years, without a definite plateau in thickness being reached until at least adolescence. The ‘soft plateau’ that we have seen in RBM thickening is also observed in normal alveolar development, whereby the exact time at which alveolar growth and multiplication ceases is unknown.20 This is the first report of a possible reduction in RBM thickness apparent during ageing, which interestingly is also mirrored in an age-associated reduction of alveolar surface area and wall tissue.20 21
This is the first description of change in epithelial height with age, and the first report of a positive relationship between RBM thickness and epithelial height. We speculate that as taller epithelial columnar cells develop, a function of the basement membrane may be to keep them anchored, and thus a thickening of the reticular component of the basement membrane may be required to help achieve this. Indeed the contact area between columnar cells and the basal lamina in subjects with asthma is significantly less than in healthy controls22 and is a proposed explanation for epithelial shedding in asthma. Increasing bronchial epithelial height with age has previously been reported from preterm until 8 months postnatal age,23 but subsequent changes in epithelial height with age were unknown. We have shown an increase in epithelial height until adolescence, with a subsequent reduction during adulthood. However, the number of adults included in our study is small, and the relationship between RBM thickness and age in adults is only weakly negative; therefore, it is difficult to draw definitive conclusions about change in RBM thickness and epithelial height with age in our adults. This relationship needs to be investigated further in future studies with a larger sample size. If confirmed, these features may be a reflection of the normal decline in lung function seen in adults. The lack of consensus about a relationship between RBM thickness and lung function may be explained by failure to take account of a natural reduction in RBM thickness in adulthood. Thus to allow accurate and meaningful interpretation of RBM data obtained in studies of endobronchial biopsies, it would seem important to include age-matched controls for children and adults.
One limitation of our current study is that we have not assessed changes in the components of the RBM with its development, which might provide clues to the important molecules and mechanisms involved. We have tried staining these formalin-fixed, paraffin wax-embedded sections for at least one RBM component, tenascin-C, but have been unsuccessful. While previous studies have reported RBM components, these have all used snap-frozen tissue, but as we only had access to paraffin sections we have been unable to explore this further. However, the importance of fibroblast growth factor in contributing to increased thickness and the role of the RBM in influencing trafficking of growth factors in the EMTU has been shown in primate airways.2 A further limitation is the use of light microscopy alone, and not additional electron microscopy to assess RBM ultrastructure. Although this may have shown the presence of a reticular layer earlier than 30 weeks gestation, such a study was not possible because all tissue was processed for clinical reasons, fixed and embedded in paraffin wax, not the resin embedding required for electron microscopy. However, we have previously reported on the ultrastructure of the RBM in non-wheezy infants, children without asthma and healthy adults, and shown that it is similar at all ages.24 We acknowledge that our postmortem lung specimens were not all fixed using the same technique, and this is why the data have been presented in three separate age groups, according to the origin of the tissue. Finally, we accept there is inequality in the number of subjects in each age group, with only small numbers available at some ages, especially the older children and adults. However, the nature of the tissue is such that we were unable to have an equal age distribution. We also acknowledge that the three age groups are made up of subjects from different areas, and that these are not longitudinal data. Despite the above limitations, we consider the large number of subjects, whose deaths were not respiratory related, and the wide age range included provides a strong basis for establishing the time of appearance and pattern of thickening that occurs to the bronchial RBM, and its positive relationship with epithelial height, during normal development. These data indicate that future paediatric and adult biopsy studies assessing RBM thickness should include age-matched controls.
Funding The British Paediatric Respiratory Society, Asthma UK, The Wellcome Trust, UK. SS is funded by an Intermediate Clinical Fellowship from the Wellcome Trust. AJ is supported by a National Health and Medical Research Council of Australia Practitioner Fellowship.
Competing interests None.
Ethics approval This study was conducted with the approval of the local ethics committees at each centre from which tissue was obtained.
Provenance and peer review Not commissioned; externally peer reviewed.
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