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Mechanisms of lung infection in the community and hospital setting
S86 Effects of exposure to IL-13, IL-31 and an IL-13/31 combination on mucociliary differentiation of bronchial epithelial cells from normal children
  1. J C Parker1,
  2. S T Thavagnanam1,
  3. G Skibinski2,
  4. L G Heaney1,
  5. M D Shields1
  1. 1Centre for Infection and Immunity, Queen's University Belfast, Belfast, UK
  2. 2School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast, Belfast, UK

Abstract

Background Asthma is characterised by airway remodelling which includes smooth muscle hypertrophy, goblet cell hyperplasia and subsequent mucus hyper-secretion. Th2 cytokines including IL-13 and more recently IL-31 have been implicated in the pathogenesis of asthma.

Objectives We aimed to examine the effects of IL-13 (20 ng/ml), IL-31 (20 ng/ml) and an IL-13/31 combination (20 ng/ml of both) on the in vitro mucociliary differentiation of paediatric bronchial epithelial cells (PBECs) from normal patients.

Hypothesis We hypothesised that cells from normal children exposed to IL-13, IL-31 or an IL-13/31 combination would alter their phenotype towards that of an asthmatic epithelium.

Methods Markers of differentiation, real time PCR for MUC5AC, MUC5AC ELISA and transepithelial electrical resistance (TEER) were assessed.

Results We found that well-differentiated paediatric bronchial epithelial cells highly expressed the IL-31 receptor (IL-31RA). Transepithelial electrical resistance (TEER) indicated good formation of tight junctions which was found to be similar across all treatment groups. We found that IL-13 stimulation reduced the number of ciliated cells compared with control (IL-13 stimulation: mean=4.8% (SD=2.5); Control: mean=18.1%, (SD=5.9)). We did not find that the combination of IL-13 and IL-31 had any additional effects to that of IL-13 alone (IL-13/31 combination stimulation: mean=5.1% (SD=4.6); Control: mean=18.1%, (SD=5.9)). Stimulation with IL-13, IL-31 and the IL-13/IL-31 combination did not result in any changes of goblet cell numbers.

Conclusions IL-31RA receptor is present in abundance in well-differentiated paediatric bronchial epithelial cells however IL-31 does not exhibit any detrimental effects on mucociliary differentiation or proliferation. In addition, IL-31 does not appear to have a synergistic effect when combined in culture with IL-13, in the differentiation process.

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