NOX4/NADPH oxidase expression is increased in pulmonary fibroblasts from patients with idiopathic pulmonary fibrosis and mediates TGFβ1-induced fibroblast differentiation into myofibroblasts
- Nadia Amara1,
- Delphine Goven1,
- Fabienne Prost1,
- Rachel Muloway1,
- Bruno Crestani1,2,
- Jorge Boczkowski3,4,5
- 1INSERM, Unité 700, Université Paris 7 Denis Diderot, site Bichat, Paris, France
- 2Assistance Publique-Hôpitaux de Paris, Service de Pneumologie A, Hôpital Bichat, Paris, France
- 3INSERM, Unité U955, Université Paris Est, Faculté de Médecine, Créteil, F-94010, France
- 4Assistance Publique-Hôpitaux de Paris, Service d'Explorations Fonctionnelles, Hôpital Henri Mondor, Créteil, France
- 5Hôpital Intercommunal de Créteil, Service de Pneumologie et Pathologie Professionnelle, Créteil, France
- Correspondence to Jorge Boczkowski, INSERM U955, Faculté de Médecine Paris 12, 94000 Créteil, France;
- Received 7 January 2009
- Accepted 25 May 2010
Background Persistence of myofibroblasts is believed to contribute to the development of fibrosis in idiopathic pulmonary fibrosis (IPF). Transforming growth factor β1 (TGFβ1) irreversibly converts fibroblasts into pathological myofibroblasts, which express smooth muscle α-actin (α-SMA) and produce extracellular matrix proteins, such as procollagen I (α1). Reactive oxygen species produced by NADPH oxidases (NOXs) have been shown to regulate cell differentiation. It was hypothesised that NOX could be expressed in parenchymal pulmonary fibroblasts and could mediate TGFβ1-stimulated conversion of fibroblasts into myofibroblasts.
Methods Fibroblasts were cultured from the lung of nine controls and eight patients with IPF. NOX4, α-SMA and procollagen I (α1) mRNA and protein expression, reactive oxygen species production and Smad2/3 phosphorylation were quantified, in the absence and in the presence of incubation with TGFβ1. Migration of platelet-derived growth factor (PDGF)-induced fibroblasts was also assessed.
Results It was found that (1) NOX4 mRNA and protein expression was upregulated in pulmonary fibroblasts from patients with IPF and correlated with mRNA expression of α-SMA and procollagen I (α1) mRNA; (2) TGFβ1 upregulated NOX4, α-SMA and procollagen I (α1) expression in control and IPF fibroblasts; (3) the change in α-SMA and procollagen I (α1) expression in response to TGFβ1 was inhibited by antioxidants and by a NOX4 small interfering RNA (siRNA); (4) NOX4 modulated α-SMA and procollagen I (α1) expression by controlling activation of Smad2/3; and (5) NOX4 modulated PDGF-induced fibroblast migration.
Conclusion NOX4 is critical for modulation of the pulmonary myofibroblast phenotype in IPF, probably by modulating the response to TGFβ1 and PDGF.
BC and JB shared senior authorship.
Funding NA was supported by Chancellerie des Universités de Paris (legs Poix), and JB by INSERM and Assistance Publique-Hôpitaux de Paris (Contrat d'Interface). This work was supported by the European Commission (FP 7, European IPF Network) and by the Agence Nationale de la Recherche (ANR Physio 2006, FIBROPNEUMO).
Competing interests None.
Patient consent Obtained.
Ethics approval This study was approved by the local ethics committee of Saint Germain en Laye Nospital and stored biopsies were reported to our institutional board (Délégation à la Recherche Clinique, Assistance Publique-Hôpitaux de Paris, Carré Historique de l'Hôpital Saint- Louis, Paris, France).
Provenance and peer review Not commissioned; externally peer reviewed.