Background: Thirteen studies investigating the association between asthma during pregnancy and perinatal mortality reported generally no increased risk. Most of these studies should be interpreted with caution because they were limited in terms of statistical power. A study was therefore undertaken to evaluate whether maternal asthma during pregnancy increases the risk of perinatal mortality.
Methods: Through three administrative databases from Québec (Canada), a cohort of women with and without asthma who had at least one pregnancy between 1990 and 2002 was formed. Perinatal mortality was identified by diagnostic codes. The adjusted odds ratio (OR) of perinatal mortality in women with and without asthma was compared using Generalised Estimation Equation (GEE) models. The first model included all potential confounders (except small for gestational age, SGA), the second model excluded birth weight, gestational age at birth and SGA and the third model excluded birth weight, gestational age at birth but included only SGA. This analysis was also stratified for birth weight and gestational age at birth.
Results: The cohort was formed of 13 100 and 28 042 single pregnancies in women with and without asthma. The crude OR of perinatal mortality was 1.35 (95% CI 1.08 to 1.67), which decreased to 0.93 (95% CI 0.75 to 1.17) after adjustment for birth weight and gestational age at birth. Women with asthma had a higher rate of low birthweight babies and preterm delivery than those without asthma.
Conclusion: The increased risk of low birthweight babies and premature delivery in women with asthma may partly explain the association between maternal asthma and the increased risk of perinatal mortality.
Statistics from Altmetric.com
Asthma is considered the most prevalent medical condition to complicate pregnancy.1 Around 4–8% of pregnancies are complicated by asthma.1 Most studies that have evaluated the effect of maternal asthma on perinatal mortality suggest that it does not significantly increase the risk,2–12 but Gordon et al13 reported a statistically significant increased risk of perinatal mortality among 273 women with asthma compared with 30 861 non-asthmatic women (5.9% vs 3.2%, p<0.05). It is worth noting, however, that the conclusion of this study was based on crude estimates. The risk of stillbirth2 8 14–21 and neonatal mortality2 8 14–17 20 associated with maternal asthma has been evaluated in several studies but no statistically significant increased risk was reported, except in the study by Bahna et al.2 Moreover, most of the negative studies should be interpreted with caution owing to small sample size and lack of power3–11 that limits the capacity of the studies to conclude the absence of an association with a reasonable degree of certainty. Among the negative studies, Tata et al21 were the only authors who performed a study with a power of >80% to detect the observed relative risk.
In order to better quantify the association between maternal asthma and perinatal mortality, we conducted a large cohort study of 41 142 pregnancies in women with and without asthma using the health administrative databases of the Canadian province of Québec.
Source of data
Our cohort of pregnancies was constructed through the linkage of three health administrative databases from Québec, Canada. The administrative database of the Régie de l’assurance-maladie du Québec (RAMQ) provides information related to the medical services dispensed to all residents of Québec as well as information related to medication prescriptions filled at community pharmacies for those residents who are covered by the Quebec’s Public Prescription Drug Insurance Plan, which represents approximately 43% of the population. The MED-ECHO database contains information on all hospital admissions in Québec. Administered by the Institut de la statistique du Québec (ISQ), the Fichier des événements démographiques provides information on all births and perinatal deaths.
We constructed a cohort formed of singleton pregnancies of all women with asthma and a 33% random sample of women without asthma who had at least one delivery (live birth or stillbirth) between 1990 and 2002, were covered by the Quebec’s Public Prescription Drug Insurance Plan for at least 1 year before conception and during pregnancy, and were aged 13–50 years at conception. Pregnancies were identified using diagnostic and act codes related to prenatal care, pregnancy complications and delivery care. The calendar date of the conception, the first day of the last menstrual period, was retrospectively calculated by subtracting the gestational age from the offspring’s date of birth as recorded in the MED-ECHO and RAMQ databases. These databases have been used in the past for epidemiological research in the field of asthma.22
The main exposure was maternal asthma. A woman was considered to have asthma if she had filled at least one prescription for an asthma medication and had at least one diagnosis of asthma in accordance with the International Classification of Diseases, 9th revision (ICD-9) codes 493.0, 493.1, 493.9, 2 years before or during her pregnancy. Once a woman was identified as having asthma, her status was kept unchanged for all subsequent pregnancies. The medical diagnosis for asthma recorded in the RAMQ Medical Services database has been formally evaluated and found to be valid.23 A women was considered to be non-asthmatic if she filled no prescription for an asthma medication between 1988 and 2002 and had no diagnosis for asthma during the entire follow-up period.
Definition of outcomes
Perinatal mortality included stillbirth and neonatal mortality. Stillbirth was defined as fetal loss beyond 20 weeks of gestation or, if the gestational age is unknown, a birth weight of ⩾500 g which corresponds to 22 weeks of gestation in a normally developing fetus. Stillbirths were identified with ICD-9 codes 656.4, V27.1, V27.3, V27.4, V27.6, V27.7, V32, V35, V36 recorded in the RAMQ, MED-ECHO or birth and death certificate databases. Neonatal mortality was defined as the death of a liveborn neonate within the first 29 days of life. Deaths occurring within the first 29 days of life were identified from the ISQ or RAMQ databases.
Potential confounding variables
Three categories of variables identified in the literature—including maternal characteristics, pregnancy-related variables and fetal characteristics—were considered as potential confounders. Maternal characteristics included the age at conception (<18 years, 18–34 years, ⩾35 years), receiving benefits from Quebec’s Social Assistance Program during the pregnancy (yes/no) and the level of education during the pregnancy (⩽11 years ⩾12 years, missing values). The pregnancy-related variables included parity (first/second or more pregnancy), pregnancy induced hypertension (PIH) (yes/no), diabetes mellitus (yes/no), gestational diabetes (yes/no), placental abruption (yes/no) and infection of amniotic cavity (yes/no). Using specific algorithms that we developed for each condition, PIH, gestational diabetes and diabetes mellitus were identified based upon either a diagnosis or a filled prescription for a related medication as recorded in the RAMQ or MED-ECHO databases 1 year before or during the pregnancy. These variables have been defined previously24 and the algorithms are available on request. Fetal characteristics included cord around the neck (yes/no) based on a diagnosis recorded in the RAMQ or MED-ECHO databases, as well as birth weight (⩽2500 g/>2500 g), gestational age at birth (<37 weeks/⩾37 weeks) and small for gestational age (SGA) (yes/no). Algorithms to measure birth weight and gestational age at birth from the data available in the MED-ECHO and ISQ databases have been developed and found to be highly valid when compared with data recorded in the mother’s medical chart.25
To compare the risk of perinatal mortality between women with and without asthma, Generalised Estimation Equation (GEE) models with a logistic link were used and potential confounders were included in the models. GEE models were used to estimate the correlation between pregnancies from the same women and to provide valid variance estimates. Seven models were used: (1) a crude model including only the maternal asthma variable (main exposure); (2) a full and a reduced model including the maternal asthma variable and considering all potential confounding variables except SGA; (3) a full and a reduced model including the maternal asthma variable and considering all potential confounding variables except birth weight, gestational age at birth and SGA; (4) a full and a reduced model including the maternal asthma variable and considering all potential confounding variables except birth weight and gestational age.
Since maternal asthma has been found to increase the risk of having a low birthweight baby,2 5 17 premature delivery2 17 26 and perinatal death,27 28 we performed a stratified analysis to further investigate the role of these variables in the causal pathway between maternal asthma and perinatal mortality. The association between maternal asthma and perinatal mortality was therefore separately studied in women who had a baby with a birth weight ⩽2500 g or born before the 37th week of gestation and in women who had a baby with a birth weight >2500 g and born at 37 weeks of gestation or more. These analyses were adjusted for all potential confounding variables listed above except SGA.
A backward selection strategy was used to select the set of confounders. This strategy consists of eliminating one by one the variables that do not act as confounders in the model, starting with all potential confounding variables in the model. When a variable was excluded from the model and the odds ratio of asthma changed by more than 10%, we considered it to be a confounder and it was reintroduced into the model. The final model was obtained when no variable could be removed from the model without modifying the odds ratio of maternal asthma by 10% or more. Analyses were performed using SAS Version 9.
The cohort was formed of 41 142 singleton pregnancies, 13 100 in women with asthma and 28 042 in women without asthma. The rates of stillbirth and perinatal mortality in women with asthma were 0.56% and 1.04%, respectively, compared with 0.47% and 0.77% in women without asthma.
The characteristics of the pregnant women with and without asthma included in the cohort are shown in table 1. The unit of analysis is the pregnancy, since 23% of the women contributed more than one pregnancy in the analysis. Women with asthma were more likely to have a low birthweight baby and a preterm delivery than those without asthma. Table 1 also shows the prevalence of perinatal mortality as a function of each covariable: women who had a placental abruption, an infection of the amniotic cavity, a low birthweight baby and a premature delivery had a greater risk of perinatal mortality.
The results of the GEE models that were used to investigate the risk of perinatal mortality between women with and without asthma, including all potential confounding variables except SGA, are shown in table 2. A statistically significant crude increased risk of perinatal mortality was observed among women with asthma compared with those without asthma (OR1.35 (95% CI 1.08 to 1.67)). After adjusting for all potential confounders, the OR decreased to 0.93 (95% CI 0.75 to 1.17). The reduced model revealed that only birth weight and gestational age at birth acted as confounders in the association between asthma and perinatal mortality. It is also worth noting that low birth weight and preterm birth were found to be very strong crude predictors of perinatal mortality with significant ORs of 34.8 and 30.6, but these ORs reduced to 9.11 and 7.07, respectively, after adjusting for maternal asthma. On the other hand, the full and the reduced models excluding birth weight, gestational age at birth and SGA showed that women with asthma tended to be more at risk of perinatal mortality than women without asthma (adjusted OR 1.25 (95% CI 0.98 to 1.58) in the full model and adjusted OR 1.30 (95% CI 1.05 to 1.57) in the reduced model). The reduced model showed that only placental abruption (OR 7.24) acted as a confounder. Similar results were found in the full and reduced models including SGA as a potential confounder while excluding birth weight and gestational age at birth (OR for maternal asthma 1.17 (95% CI 0.91 to 1.59) in the full model and 1.22 (95% CI 0.98 to 1.53) in the reduced model). Only SGA (OR 2.21) and placental abruption (OR 7.41) were found to act as confounders in this reduced model.
The results of the stratified analysis are shown in table 3. In the first stratum formed of normal weight (>2500 g) and term babies (⩾37 weeks) there were 11 283 and 25 552 pregnancies in women with and without asthma, respectively. In this stratum, after adjustment for all potential confounders, women with asthma were not found to be at increased risk of perinatal mortality (OR 0.97 (95% CI 0.74 to 1.29)) compared with women without asthma. In the second stratum formed of babies with a birth weight <2500 g or born before the 37th week of gestation, there were 1817 and 2490 pregnancies in women with and without asthma, respectively. In this stratum, asthma was also not found to be associated with an increased risk of perinatal mortality (OR 0.94 (95% CI 0.73 to 1.22)).
In this study we observed a crude significant increased risk of perinatal mortality of 35% among women with asthma compared with women without asthma that did not remain after adjustment for birth weight and gestational age at birth. However, models excluding these variables or adjusted for SGA reveled that women with asthma tended to be more at risk of perinatal mortality than those without asthma. A stratified analysis showed that, among normal weight and term babies, maternal asthma during pregnancy did not increase the risk of perinatal death. Moreover, the proportions of low birthweight and preterm babies were higher in women with asthma than in those without asthma, and birth weight and gestational age at birth were very strong predictors of perinatal mortality. These results suggest that birth weight and gestational age at birth are likely to be in the causal pathway between asthma and perinatal mortality.
Twenty previously published studies investigated the association between maternal asthma and stillbirth, neonatal mortality and/or perinatal mortality.2–21 A significant increased risk of neonatal mortality and perinatal mortality in women with asthma was reported in only two studies,2 13 but 10 of the studies2 6 9 10 12–16 19 reported only crude estimates. Gordon et al13 were the only authors to report a statistically significant increased risk of perinatal mortality among 273 (5.9%) women with asthma compared with 30 861 (3.2%) non-asthmatic women (p<0.05), and only Bahna et al2 observed a significantly higher rate of neonatal mortality among 381women with asthma compared with 112 530 women with no recorded disease before and during pregnancy (p<0.05). These studies were performed in 1970 and 1972 at a time when neonatal intensive care was probably less sophisticated and fetal surveillance less intensive, explaining the high observed rate of perinatal mortality. Nevertheless, the crude relative risk estimate of perinatal mortality (1.84) is comparable to the crude OR of perinatal mortality (1.35) found in our study, both of them being statistically significant.
Ten of these 20 studies3–5 7 8 11 17 18 20 21 reported estimates that were adjusted or matched for one or several confounding variables such as maternal age, body mass index (BMI) before pregnancy, parity, multiple pregnancy, smoking status, year of delivery, gestational diabetes, pre-existing diabetes, pre-existing hypertension, rupture of membrane and infection of amniotic cavity, and none of them found a significant increased risk of of stillbirth, neonatal mortality or perinatal mortality. The largest of these studies21 found that the risk of stillbirth was similar in 37 585 pregnancies in women with asthma as in 243 434 pregnancies in women without asthma after adjusting for maternal age, smoking status and BMI before pregnancy, year of birth, multiple pregnancy and sex of child (OR 1.04). None of these studies adjusted their results for birth weight or gestational age at birth.
Women with asthma during pregnancy have been found to be more at risk of having babies of low birth weight,2 17 premature deliveries2 17 26 and placental abruption.26 Moreover, asthma exacerbations requiring hospitalisation during pregnancy, maternal asthma symptoms and poor asthma control have been associated with an increased risk of low birthweight babies,7 SGA babies15 and fetal growth restriction.29 In addition, these adverse perinatal and pregnancy outcomes have been found to increase the risk of perinatal mortality.27 28 30 Biological mechanisms could explain the relationship between maternal asthma and low birthweight and preterm babies. Fetal hypoxia (possibly due to poor asthma control) has been suggested as a mechanism for the association between asthma and intrauterine growth restriction.17 A hypoxic process at the maternal/fetal interface related to maternal asthma was also proposed to lead to placental abruption.31 Moreover, increased inflammatory cytokines and increased smooth muscle reactivity found in subjects with asthma may be associated with premature delivery.9 These data and those of Gordon et al13 on the association between severe asthma and perinatal mortality suggest that adequate control of asthma during pregnancy could reduce the risk of having low birthweight and preterm babies and, as a consequence, might reduce the risk of perinatal deaths in infants of women with asthma.
Our crude OR showed an increased risk of perinatal mortality among women with asthma, but our OR adjusted for birth weight and gestational age at birth and the ORs found in the stratified analysis showed no impact of maternal asthma during pregnancy on perinatal mortality. Indeed, in the stratified analysis we found no increased risk of perinatal mortality associated with asthma among normal weight and term babies and among low birthweight or preterm babies. The fact that there was a crude effect of asthma on perinatal mortality, that the effect vanished after adjusting for birth weight and gestational age at birth, that there is a higher prevalence of low birthweight and preterm babies among women with asthma, that birth weight and gestational age at birth are very strong predictors of perinatal mortality and that there was no effect of asthma on normal weight and term babies suggest that asthma mainly increases the risk of perinatal mortality through lowering the weight of the baby and increasing the risk of a preterm delivery.
This study has several strengths. Using the administrative databases of Quebec presents certain advantages since they provided a large sample size of pregnant women with and without asthma, as well as the possibility of adjusting the risk estimates for a wide variety of potential confounders. Moreover, the information recorded in the administrative databases is prospectively collected, independent of the outcome. In addition, the diagnoses recorded in the RAMQ databases for the identification of patients with asthma are physician-based and have been shown to be valid.23
However, this study has a few limitations that should be kept in mind when interpreting the results. Some maternal characteristics are not available in the databases, such as cigarette smoking and obesity. Studies have found that maternal smoking is associated with an increased risk of perinatal mortality,32 and women with asthma might be more likely to smoke than those without asthma.8 11 Not adjusting for smoking could therefore result in unadjusted confounding. However, it is expected that a large part of the impact of cigarette smoking would be captured by the variables birth weight, gestational age at birth and placental abruption that are included in our model. Indeed, the increased risk of stillbirth and perinatal mortality among smoking mothers is generally explained by fetal growth restriction, very early delivery and placenta abruption.32 33 Maternal smoking is associated with fetal hypoxia due to the reduction of the uteroplacental blood flow, and the increase in vascular resistance may partly explain the association between smoking and reduced fetal growth and also may contribute to the increased risk of placental abruption.34
Obesity might act as a confounder in the association between maternal asthma and perinatal mortality since obesity has been found to be more prevalent in women with asthma than in those without asthma,35 and obesity has been associated with an increased risk of stillbirth.36 Not adjusting for obesity in our study might have overestimated the impact of asthma on perinatal mortality.
In addition, the RAMQ databases provide medication information only for welfare recipients and residents who are covered by Quebec’s Public Prescription Drug Insurance Plan; residents who are covered by a private drug insurance plan are excluded. This led to a sample that was under-represented by women with a high socioeconomic status. Socioeconomic status has been associated with an increased risk of perinatal mortality.37 Thus, generalisation of our results may be limited to women of lower socioeconomic status.
In conclusion, the increased risk of a low birthweight baby and premature delivery among women with asthma may partly explain the association between maternal asthma and the increased risk of perinatal mortality.
See Editorial, p 93
Funding: This project was funded by grants from the Fonds de la recherche en santé du Québec (FRSQ) and Canadian Institute for Health Research (CIHR). LB and M-FB are co-chair of the Endowment Pharmaceutical Chair AstraZeneca in Respiratory Health. LB is the recipient of a Junior II investigator salary support from the FRSQ. M-CB is the recipient of a doctoral research scholarship of the FRSQ and the Conseil du médicament du Québec. CL is the recipient of a New Investigator Salary Award from the CIHR.
Competing interests: None.