Article Text

PDF

Prolonged survival of neutrophils from patients with ΔF508 CFTR mutations
  1. D J McKeon1,
  2. A M Condliffe1,
  3. A S Cowburn1,
  4. K C Cadwallader1,
  5. N Farahi2,
  6. D Bilton1,
  7. E R Chilvers1
  1. 1
    Respiratory Medicine Division, Department of Medicine, University of Cambridge School of Clinical Medicine, Addenbrooke’s Hospital, Cambridge, UK
  2. 2
    Adult CF Centre, Papworth Hospital, Cambridge, UK
  1. Professor E R Chilvers, Respiratory Medicine Division, Department of Medicine, University of Cambridge School of Clinical Medicine, Box 157, Addenbrooke’s Hospital, Hills Road, Cambridge CB2 2QQ, UK; erc24{at}cam.ac.uk

Statistics from Altmetric.com

Cystic fibrosis (CF) is an autosomal recessive “channelopathy” characterised by aberrant CFTR and ENaC function resulting in widespread epithelial cell dysfunction and persistent airway infection. Studies indicating that airway inflammation precedes infection1 and that patients with CF display exaggerated neutrophilic responses to pathogens have suggested a primary defect in innate immune responses in CF. Given the importance of apoptosis to the resolution of neutrophilic inflammation,2 we sought to determine whether circulating CF neutrophils display normal apoptotic capacity.

Peripheral blood neutrophils were isolated from 12 clinically stable Pseudomonas colonised ΔF508 homozygote adult patients with CF and 12 age and sex matched healthy controls using discontinuous plasma/Percoll gradients.3 Blood neutrophil counts were in the normal range for all subjects (CF mean 4.1 (range 5.4–8.3), controls 2.5 (3.7–6.6) ×109/l); none of the patients with CF were taking azithromycin. CF and control neutrophils were isolated in parallel and handled identically. Cells were cultured (5×106/ml, 5% CO2, 37°C) in Iscove’s Dulbecco’s medium with 10% autologous serum for 6 and 20 h, as detailed previously.3 These incubations were conducted with or without tumour necrosis factor α (TNFα 10 ng/ml) or granulocyte macrophage-colony stimulating factor (GM-CSF 10 ng/ml), which have defined proapoptotic and antiapoptotic effects, respectively.3 Apoptosis was assessed by blinded morphological assessment of May–Gründwald–Giemsa stained cytospins, confirmed by quantification of annexin-V–fluorescein isothiocyanate binding and propidium iodide staining.3

Neutrophils from patients with CF had delayed constitutive apoptosis and were resistant to the normal early proapoptotic effects of TNFα,3 yet remained fully sensitive to the antiapoptotic effects of GM-CSF (fig 1A–C). Incubation of control neutrophils with 10% serum from patients with CF mimicked exactly the enhanced survival effect seen in CF cells, suggesting an acquired rather than intrinsic defect (fig 1D). These data indicate that blood neutrophils from clinically well patients with CF display a relative resistance to spontaneous and death ligand induced apoptosis when cultured with autologous serum. Using the Luminex FlowMetrix system to compare protein and cytokine profiles in CF and control sera, only C reactive protein (CRP) differed, with a 3.6-fold increase in CF sera (controls 1.6 (0.3) μg/ml, CF 5.8 (1.3) μg/ml; p<0.05, n = 4). Although no direct measures of neutrophil activation were performed, there were no differences in proinflammatory cytokines or growth factors present in control and CF sera, including interleukin (IL)8, TNFα, IL6 and GM-CSF. Previous data reporting that migration inhibitory factor can inhibit CF neutrophil apoptosis may reflect lipopolysaccharide contamination of the cytokine preparation.4 Moreover, the delay in constitutive apoptosis in CF neutrophils was inhibited by LY294002 (10 μM), a phosphoinositide 3-kinase (PI3 kinase) inhibitor (% apoptosis 20 h: control 54 (2), control+LY294002 61 (5), CF 33 (7), CF+LY294002 50 (9), n = 3).

Figure 1 Human peripheral blood neutrophils from patients with cystic fibrosis (CF) and matched control subjects were incubated for 6 or 20 h in DMEM containing 10% autologous serum (unless otherwise stated) and then assessed for apoptosis using morphological criteria. (A) Delay in constitutive apoptosis in CF neutrophils at 6 and 20 h (*p<0.05, n = 12). (B) Loss of the early proapoptotic effect of tumour necrosis factor α (T) at 6 h (*p<0.05, n = 5). (C) Preserved prosurvival effect of granulocyte macrophage-colony stimulating factor (G) in CF neutrophils (*p<0.05, n = 11). (D) Ability of sera from patients with CF to delay apoptosis in normal neutrophils (*p<0.05, n = 4). Parallel assessment of apoptosis using annexin-V– fluorescein isothiocyanate binding and propidium iodide staining3 resulted in essentially identical data (note shown). Data are expressed as mean (SEM) of (n) separate experiments, each conducted in triplicate and analysed using non-parametric (Mann–Whitney) calculations of significance.

These findings add to the body of data suggesting broader defects in innate immune responses in CF. Factors present in CF serum appear to inhibit both constitutive and TNFα induced apoptosis, which would be predicted to impair the physiological removal of these cells at inflamed sites. A potential role for CRP is supported by reports that monomeric CRP, which is generated in inflamed tissues, can inhibit neutrophil apoptosis via a mechanism involving activation of FcγRIII (CD16) and PI3 kinase.5 Together, these results suggest that CF neutrophils have an impaired capacity to undergo apoptosis, even prior to migration to the lung.

REFERENCES

View Abstract

Footnotes

  • Funding: Funded by the CF Trust UK (DJM), Wellcome Trust (AMC), Asthma-UK (ASC, NF), Papworth Hospital and BLF (KCC).

  • Competing interests: None.

  • Ethics approval: Ethics approval was obtained.

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.