β-Actin and GAPDH housekeeping gene expression in asthmatic airways is variable and not suitable for normalising mRNA levels

E M Glare; M Divjak; M J Bailey; E H Walters

doi:10.1136/thorax.57.9.765

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β-Actin and GAPDH housekeeping gene expression in asthmatic airways is variable and not suitable for normalising mRNA levels

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	Abstract	Full	Pdf
Aug 2002	46	23	18
Sep 2002	32	194	52
Oct 2002	6	140	74
Nov 2002	6	90	35
Dec 2002	4	60	17
Jan 2003	6	77	23
Feb 2003	13	48	20
Mar 2003	16	88	30
Apr 2003	4	58	27
May 2003	2	52	16
Jun 2003	9	76	17
Jul 2003	27	81	21
Aug 2003	23	56	19
Sep 2003	16	56	24
Oct 2003	38	71	24
Nov 2003	23	43	56
Dec 2003	18	51	32
Jan 2004	39	74	33
Feb 2004	20	71	32
Mar 2004	21	66	41
Apr 2004	18	54	23
May 2004	56	80	34
Jun 2004	48	61	17
Jul 2004	50	80	38
Aug 2004	53	72	57
Sep 2004	82	83	36
Oct 2004	52	78	43
Nov 2004	65	54	61
Dec 2004	57	57	31
Jan 2005	66	48	30
Feb 2005	57	92	38
Mar 2005	69	78	66
Apr 2005	51	62	46
May 2005	31	69	67
Jun 2005	41	69	54
Jul 2005	39	81	63
Aug 2005	38	97	60
Sep 2005	36	89	49
Oct 2005	73	76	59
Nov 2005	74	86	59
Dec 2005	51	75	54
Jan 2006	73	98	50
Feb 2006	38	93	43
Mar 2006	66	92	63
Apr 2006	64	91	65
May 2006	99	60	47
Jun 2006	57	57	37
Jul 2006	46	63	33
Aug 2006	77	73	41
Sep 2006	92	64	45
Oct 2006	98	90	75
Nov 2006	60	74	53
Dec 2006	54	37	34
Jan 2007	76	55	48
Feb 2007	42	56	42
Mar 2007	58	93	61
Apr 2007	59	36	24
May 2007	50	31	25
Jun 2007	69	48	42
Jul 2007	52	24	21
Aug 2007	53	17	23
Sep 2007	45	22	20
Oct 2007	55	24	23
Nov 2007	47	26	21
Dec 2007	33	21	21
Jan 2008	63	31	23
Feb 2008	87	28	30
Mar 2008	81	30	30
Apr 2008	88	20	35
May 2008	72	21	16
Jun 2008	54	28	15
Jul 2008	57	23	17
Aug 2008	71	33	27
Sep 2008	68	27	22
Oct 2008	65	28	15
Nov 2008	66	24	11
Dec 2008	47	27	14
Jan 2009	65	23	8
Feb 2009	61	26	15
Mar 2009	72	31	18
Apr 2009	82	32	16
May 2009	63	36	12
Jun 2009	59	37	13
Jul 2009	60	28	9
Aug 2009	64	28	19
Sep 2009	66	28	15
Oct 2009	100	31	15
Nov 2009	86	21	14
Dec 2009	66	13	8
Jan 2010	74	18	10
Feb 2010	67	33	7
Mar 2010	113	55	19
Apr 2010	105	55	17
May 2010	75	38	22
Jun 2010	73	27	6
Jul 2010	50	33	10
Aug 2010	40	36	14
Sep 2010	62	32	11
Oct 2010	81	36	15
Nov 2010	69	35	16
Dec 2010	53	30	8
Jan 2011	53	28	9
Feb 2011	60	30	14
Mar 2011	86	43	18
Apr 2011	61	37	17
May 2011	88	25	13
Jun 2011	61	22	11
Jul 2011	61	19	4
Aug 2011	59	34	19
Sep 2011	89	24	6
Oct 2011	98	28	13
Nov 2011	96	65	21
Dec 2011	39	104	29
Jan 2012	45	89	29
Feb 2012	43	58	31
Mar 2012	42	77	74
Apr 2012	40	85	52
May 2012	53	89	50
Jun 2012	40	80	55
Jul 2012	51	70	60
Aug 2012	34	49	60
Sep 2012	60	40	68
Oct 2012	82	115	68
Nov 2012	69	84	56
Dec 2012	54	100	62
Jan 2013	54	140	101
Feb 2013	46	104	46
Mar 2013	66	160	80
Apr 2013	65	194	108
May 2013	48	229	111
Jun 2013	31	135	47
Jul 2013	45	171	71
Aug 2013	57	134	118
Sep 2013	50	168	114
Oct 2013	60	200	138
Nov 2013	39	190	154
Dec 2013	59	154	84
Jan 2014	69	261	126
Feb 2014	53	256	119
Mar 2014	69	406	173
Apr 2014	96	459	178
May 2014	62	499	129
Jun 2014	61	482	161
Jul 2014	62	482	120
Aug 2014	47	389	90
Sep 2014	47	401	94
Oct 2014	56	122	60
Nov 2014	86	77	31
Dec 2014	61	112	20
Jan 2015	59	143	72
Feb 2015	74	90	32
Mar 2015	98	442	52
Apr 2015	47	511	61
May 2015	87	506	74
Jun 2015	52	460	47
Jul 2015	63	325	79
Aug 2015	39	385	48
Sep 2015	54	382	42
Oct 2015	37	261	27
Nov 2015	43	401	48
Dec 2015	64	347	41
Jan 2016	125	377	49
Feb 2016	13	389	37
Mar 2016	25	473	64
Apr 2016	32	501	52
May 2016	25	458	51
Jun 2016	44	424	55
Jul 2016	46	456	80
Aug 2016	44	397	51
Sep 2016	46	322	50
Oct 2016	62	497	66
Nov 2016	73	469	82
Dec 2016	104	401	43
Jan 2017	49	369	45
Feb 2017	39	215	41
Mar 2017	35	270	58
Apr 2017	34	352	54
May 2017	33	433	50
Jun 2017	80	289	51
Jul 2017	100	82	62
Aug 2017	86	49	92
Sep 2017	233	181	217
Oct 2017	369	309	160
Nov 2017	358	254	169
Dec 2017	252	186	100
Jan 2018	236	182	164
Feb 2018	221	175	108
Mar 2018	295	243	106
Apr 2018	354	284	147
May 2018	371	289	104
Jun 2018	302	272	103
Jul 2018	267	247	137
Aug 2018	343	309	76
Sep 2018	349	321	121
Oct 2018	383	353	182
Nov 2018	389	325	136
Dec 2018	290	264	97
Jan 2019	342	281	85
Feb 2019	340	298	78
Mar 2019	388	337	121
Apr 2019	468	396	121
May 2019	473	407	91
Jun 2019	484	424	162
Jul 2019	425	393	193
Aug 2019	377	338	150
Sep 2019	443	403	133
Oct 2019	684	633	113
Nov 2019	635	586	180
Dec 2019	513	445	157
Jan 2020	452	404	108
Feb 2020	386	334	83
Mar 2020	436	384	110
Apr 2020	312	286	124
May 2020	357	307	75
Jun 2020	452	428	74
Jul 2020	503	480	76
Aug 2020	400	379	109
Sep 2020	469	409	112
Oct 2020	529	476	95
Nov 2020	646	599	95
Dec 2020	705	654	97
Jan 2021	684	623	91
Feb 2021	687	605	133
Mar 2021	690	628	127
Apr 2021	649	579	104
May 2021	619	612	102
Jun 2021	585	594	126
Jul 2021	458	462	87
Aug 2021	467	475	106
Sep 2021	471	480	84
Oct 2021	589	561	137
Nov 2021	248	159	93
Dec 2021	287	246	74
Jan 2022	226	198	84
Feb 2022	580	556	119
Mar 2022	902	879	161
Apr 2022	671	656	125
May 2022	724	679	154
Jun 2022	440	393	92
Jul 2022	382	349	77
Aug 2022	382	371	72
Sep 2022	415	368	85
Oct 2022	523	472	98
Nov 2022	538	455	113
Dec 2022	582	480	129
Jan 2023	449	389	86
Feb 2023	495	442	74
Mar 2023	509	412	79
Apr 2023	535	404	94
May 2023	580	460	114
Jun 2023	463	391	69
Jul 2023	418	353	73
Aug 2023	410	342	65
Sep 2023	319	266	54
Oct 2023	524	448	75
Nov 2023	691	506	100
Dec 2023	425	307	82
Jan 2024	468	202	50
Feb 2024	330	191	56
Mar 2024	378	323	88
Total	46153	55830	16794

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