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Thorax 56:306-311 doi:10.1136/thorax.56.4.306
  • Original article

Sputum induction as a research tool for sampling the airways of subjects with cystic fibrosis

Table 3

Cytokine concentrations in expectorated sputum (ES), induced sputum (SI), and bronchoalveolar lavage (BAL) fluid

ES SI BAL fluid
IL-8 (ng/ml) 18.0 (12.2) 12.8 (7.5) 4.0 (3.0)*
Urea corrected IL-8 (ng/ml) (n=8) 13.4 (11.0) 13.5 (7.1) 13.9 (17.9)
TNFα (pg/ml) 102 (101)* 45 (27) 18 (19)
Urea corrected TNFα (pg/ml) (n=8) 80 (71) 42 (42) 43 (42)
  • There was a difference in IL-8 concentration in SI from that in ES and BAL (p=0.01 and p=0.03, respectively). When a dilution correction was made these differences were no longer seen. There was a difference in TNFα concentration between SI and ES (p=0.03) but this was no longer seen when corrected for dilution.

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