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Thorax 54:439-441 doi:10.1136/thx.54.5.439
  • Short paper

Collagen degrading activity associated withMycobacterium species

Table 2

Mean (SE) collagenolytic activity of culture filtrates obtained from mycobacteria isolated from patients with tuberculosis

Diagnosis2-150 Source2-151 With Ca With EDTA
Counts per minute (cpm) Enzyme activity Counts per minute (cpm) Enzyme activity
Trypsin 792  (130) 6.3 404  (103) 3.2
Cl histolyticum 9763 (559) 78.1 2367 (362) 18.9
Pulmonary TB Sputum 1160 (284) 9.2 510 (462) 4.0
Pulmonary TB Sputum 4198 (204) 33.5 684 (642) 5.4
Pulmonary TB Pleural fluid 1783 (462) 14.2 348 (90) 2.7
Pulmonary TB Pleural fluid 1376 (248) 11.0 1142 (108) 9.1
Miliary TB Sputum 2276 (98) 18.2 874 (138) 6.9
Miliary TB Sputum 4986 (230) 39.8 566 (32) 4.5
Miliary TB Pleural fluid 2864 (127) 22.9 814 (175) 6.5
Renal TB Urine 2316 (224) 18.5 1238 (148) 9.9
  • 2-150 All patients were PPD positive.

  • 2-151 Source refers to the secretion from which the mycobacteria were isolated.

  • Background cpm have already been subtracted. The percentage of activity was calculated assuming that all the radioactivity (12 500 cpm/well) was liberated by the enzyme.

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