Table 2

Induced sputum total and differential cell count and fluid analysis (ECP and albumin) when performed (A) without and (B) with methacholine challenge

Patient no.	TCC (A)	TCC (B)	Mac (A)	Mac (B)	Neu (A)	Neu (B)	Eos (A)	Eos (B)	Lym (A)	Lym (B)	Epi (A)	Epi (B)	ECP (A)	ECP (B)	Alb (A)	Alb (B)
1	800	1600	9.8	7.6	76	79.4	13.8	12.8	0.4	0	0	0.2			664	424
2	80	384	15	22.4	83.5	76.4	1.5	0.4	0	0.4	0	0.2
3	1680	880	64.8	33.6	12.4	16.4	18	42	1.2	0.4	3.6	7.6
4	880	1840	22.6	18.4	76.2	80.2	1.2	1	0	0.2	0	0.2	624	142	232	136
5	1280	2240	55.6	31.8	38.4	64.4	5.6	3.6	0.4	0	0	0.2	960	2080	1280	1776
6	440	800	58.4	45.2	39.2	51	0.2	0.4	2	3.4	0.2	0
7	320	128	57.6	49	40.4	49.6	0	0	1	0	1	1.4	40	33	120	40
8	96	360	67.2	61.4	11.2	16.2	20.8	21	0	0	0.8	1.4
9	160	280	81.8	80.8	15.6	14.8	2.4	3.2	0.2	0.6	0	0.6	480	608	120	320
10	480	96	40.4	57.4	59.2	42	0	0.2	0	0	0.4	0.4	520	96	552	384
11	640	336	55.2	50.4	41.4	45	3	4.2	0	0.4	0.4	0	976	1104	320	728
12	480	240	62.4	51.4	28.8	42	7.2	5.6	1.6	0.8	0	0.2	1200	1520	224	632
13	480	336	77.2	73	22	26.6	0.4	0	0.4	0.2	0	0.2
14	336	880	29.4	26.4	69.2	72.8	0.8	0.4	0	0	0.6	0.4	600	416	152	216
15	800	560	62.6	68	27.8	25.4	8.4	6.4	0	0	1.2	0.2
16	312	536	42.6	44.2	5.6	3.6	51.6	52	0.2	0	0	0.2
Median	480	460	56.6	47.1	38.8	43.5	2.7	3.4	0.2	0.1	0.1	0.2	612	512	232	384
Range	80–1680	96–2240	9.8–81.8	7.6–80.8	5.6–83.5	3.6–80.2	0–51.6	0–52	0–1.6	0–3.4	0–3.6	0–7.6	40–1200	33–2080	120–1280	40–1776
Wilcoxon	p = 0.32		p = 0.08		p = 0.06		p = 0.49		p = 0.69		p = 0.22		p = 1.0		p = 0.37
R	0.56		0.83		0.93		0.91		0.71		0.73		0.66		0.81

TCC = total cell count (no. cells/ml × 10⁴); Mac = macrophages (%); Neu = neutrophils (%); Eos = eosinophils (%); Lym = lymphocytes (%); Epi = epithelial cells (%); ECP = μg/ml; albumin = μg/ml; R = coefficient of reliability.

Effect of methacholine challenge on cellular composition of sputum induction

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