Article Text

A method for evaluating the effects of allograft sterilization procedures on the viability of human fibroblasts
1. S. C. W. Chalcroft,
2. J. B. Gavin,
3. R. N. Seelye
1. Department of Pathology, University of Auckland, Auckland, New Zealand

## Abstract

Chalcroft, S. C. W., Gavin, J. B., and Seelye, R. N. (1974).Thorax, 29, 539-544. A method for evaluating the effects of allograft sterilization procedures on the viability of human fibroblasts. A semiquantitative method for assessing the effects of allograft sterilization procedures on the viability of cultured human fibroblasts is described. The proportions of viable', altered', and `non-viable' cells in four groups of fibroblasts on coverslips were estimated using light microscopy and their staining reactions with neutral red and nigrosin. While a control group of fibroblasts remained viable, exposure for one to three weeks to each of three solutions used in allograft preparation produced a progressive transition first to the altered and then to the non-viable category. These findings were confirmed by electron microscopic examination of similarly treated cells grown on millipore filters. Applications of the method are discussed.

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