IMAGES IN THORAX

^99mTechnetium-labelled neutrophil scanning in pneumonia

P Ruparelia¹, K Szczepura², C Summers¹, A M Peters², E R Chilvers¹

¹ Department of Medicine, University of Cambridge School of Clinical Medicine, Addenbrooke’s and Papworth Hospitals, Cambridge, UK
² Department of Radiology, University of Cambridge School of Clinical Medicine, Addenbrooke’s and Papworth Hospitals, Cambridge, UK

Correspondence to:
Professor E R Chilvers, Respiratory Medicine Division, Department of Medicine, University of Cambridge School of Clinical Medicine, Box 157 Addenbrooke’s Hospital, Hill’s Road, Cambridge CB2 2QQ, UK; erc24{at}cam.ac.uk

A 70-year-old man with severe smoking-related chronic obstructive pulmonary disease (COPD) was recruited to a research study to examine the trafficking of 99m-technetium (^99mTc)-labelled neutrophils. These studies were undertaken in patients with stable moderate to severe COPD and designed to establish novel methodology (using autologous ^99mTc-labelled neutrophils and single photon emission computed tomography (SPECT)) to quantify neutrophil trafficking and accumulation within the lungs of patients with COPD. At the time of scanning he was clinically stable with a C-reactive protein of 6 mg/ml and peripheral neutrophil count of 3.2x10⁹/l. He was a current smoker with a >100 pack-year history and had no other lung condition. His last COPD exacerbation was 4 months before the scan.

He was injected with 200 MBq ^99mTc-labelled autologous neutrophils and underwent SPECT scanning at 45 min, 2 and 4 h post injection using a Picker Prism 2000 gamma camera. Following image acquisition, regions of interest were drawn around the cardiac, bone marrow and lung regions. As shown in fig 1A, the 4 h scan demonstrated unexpected and intense focal activity in the left lower lobe indicative of localised uptake of ^99mTc-labelled neutrophils (a 4 h image from an age- and severity-matched current smoking subject with COPD is shown in fig 1B, demonstrating diffuse lung uptake only). Of note, at 4 h there is minimal activity present within the vascular compartment. Over the subsequent 48 h the patient developed symptoms of increased dyspnoea, cough, purulent sputum, fever and signs of focal consolidation confirmed on a chest radiograph. Moraxella catarrhalis was isolated from his sputum. The patient made a full clinical and radiological recovery following antibiotic administration.

View larger version (54K): in this window | in a new window | PowerPoint for Teaching   Figure 1 (A) Single photon emission computed tomographic (SPECT) scan taken after 4 h demonstrating focal uptake of 99m-technetium (99mTc)-labelled neutrophils in the left lower lobe (blue arrow). The red arrow depicts the expected uptake of 99mTc-labelled neutrophils in the vertebral bone marrow. Normal uptake is also seen anteriorly in the sternum and in the ribs. (B) SPECT scan of a matched subject with chronic obstructive pulmonary disease showing minimal activity within the vascular compartment at 4 h and a diffuse signal within the lung fields.

 

Previous animal studies have shown that neutrophil migration into the lung following experimental infection is rapid in both onset and offset, with migration ceasing after 6 h.¹ As a consequence, radiolabelled neutrophil imaging in patients with uncomplicated community acquired pneumonia is invariably negative.² Focal activity can, however, be seen when neutrophil activity is monitored using ¹⁸F-labelled fluorodeoxyglucose (¹⁸FDG).³ We believe this is the first image of neutrophil uptake in a patient with pneumonia obtained using radiolabelled neutrophils and SPECT.

REFERENCES

TOP REFERENCES

1. Lichter JP, Konopka RG, Hartman MT, et al.. The in vivo behaviour of granulocytes labelled with indium-111 in a canine model of pneumococcal pneumonia. Am Rev Respir Dis 1984;129:602–6.[Medline]
2. Saverymuttu SH, Phillips G, Peters AM, et al.. Indium 111 autologous leukocyte scanning in lobar pneumonia and lung abscesses. Thorax 1985;40:925–30.[Abstract/Free Full Text]
3. Jones HA, Clark RJ, Rhodes CG, et al.. In vivo measurement of neutrophil activity in experimental lung inflammation. Am J Respir Crit Care Med 1994;149:1635–9.[Abstract]

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