CHRONIC OBSTRUCTIVE PULMONARY DISEASE

Bronchial mucosal dendritic cells in smokers and ex-smokers with COPD: an electron microscopic study

A V Rogers¹, E Ädelroth², K Hattotuwa³, A Dewar⁴, P K Jeffery¹

¹ Lung Pathology Unit, Department of Gene Therapy, Imperial College London, London, UK
² Umeå University and Faculty of Medicine, Sweden
³ Respiratory Medicine, Broomfield Hospital, Chelmsford, UK
⁴ Electron Microscope Unit, NHLI, Imperial College London, London, UK

Professor P K Jeffery, Lung Pathology Unit, Royal Brompton Hospital, Sydney Street, London SW3 6NP, Chelmsford, UK; p.jeffery{at}imperial.ac.uk

Background: Bronchial mucosal dendritic cells (DCs) initiate and regulate immune responses to inhaled antigens, viruses and bacteria. Currently, little is known of their numbers in patients with chronic obstructive pulmonary disease (COPD). While reductions in their numbers have been reported recently in smokers with asthma, nothing is known of the effects of cigarette smoking on bronchial DCs in COPD. The present study compares DC numbers in smokers and ex-smokers with COPD.

Methods: Endobronchial biopsies were obtained from 15 patients with moderate to severe COPD (10 current smokers with median forced expiratory volume in 1 s (FEV₁) 45.5% predicted (range 23–68) and 5 ex-smokers with median FEV₁ 30% predicted (range 21–52)), 11 non-smokers with asthma (median FEV₁ 102% predicted (range 89–116)) and 11 non-smoker healthy controls (median FEV₁ 110% predicted (range 92–135)). Transmission electron microscopy (TEM) was used to identify the total population of DCs by their ultrastructure and their number in the epithelium and subepithelium was counted.

Results: Median (range) DC numbers were significantly lower in current smokers with COPD in the epithelium (0.0 (0.0–156.8) cells/mm²) and the subepithelium (4.5 (0.0–63.6) cells/mm²) compared with ex-smokers with COPD (97.9 (93.5–170.3) cells/mm² in the epithelium (p<0.05); 91.8 (38.2–283.3) cells/mm² in the subepithelium (p<0.01)). DC numbers in ex-smokers with COPD were similar to those in subjects with atopic asthma and healthy controls (131.6 (33.3–235.5) cells/mm² in the epithelium and 64.4 (0.0–182.4) cells/mm² in the subepithelium for the latter).

Conclusions: In COPD, bronchial mucosal DC numbers are lower in current smokers while, in those who quit, numbers are similar to non-smoking subjects with asthma and non-smoking healthy controls. The functional consequences of the reduction in mucosal DC numbers in smokers with COPD have yet to be determined.

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