HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS REGISTER		Author Keyword(s) Vol Page [Advanced]

This Article Full Text Full Text (PDF) All Versions of this Article: thx.2007.078030v1 63/2/100    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this link to a friend Similar articles in this journal Similar articles in PubMed Add article to my folders Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Barreiro, E Search for Related Content PubMed PubMed Citation Articles by Barreiro, E Topic Collections Relevant Articles

Cytokine profile in quadriceps muscles of patients with severe COPD

¹ Muscle and Respiratory System Research Unit (URMAR) and Respiratory Medicine Department, IMIM-Hospital del Mar, Centro de Investigación en Red de Enfermedades Respiratorias (CibeRes), Health and Experimental Sciences Department (CEXS), Pompeu Fabra University (UPF), Barcelona, Catalonia, Spain
² Respiratory Medicine Department, Maastricht University Hospital, Maastricht, The Netherlands
³ Respiratory Medicine Department, Royal Brompton Hospital, Imperial College, London, UK
⁴ Pneumology Department and Research Unit, Cruces Hospital, Basque Country University, Barakaldo, Basque Country, Spain

Correspondence to:
Dr E Barreiro, Muscle and Respiratory System Research Unit (URMAR), IMIM, C/Dr Aiguader, 88, Barcelona, E-08003 Spain; ebarreiro{at}imim.es

Background: Systemic proinflammatory cytokines and oxidative stress have been described in association with peripheral muscle wasting and weakness of patients with severe chronic obstructive pulmonary disease (COPD), but their expression in skeletal muscle is unknown. The objectives of the present study were to determine muscle protein levels of selected cytokines in patients with COPD and to study their relationships with protein carbonylation as a marker of oxidative stress, quadriceps function and exercise capacity.

Methods: We conducted a cross sectional study in which 36 cytokines were detected using a human antibody array in quadriceps specimens obtained from 19 patients with severe COPD and seven healthy controls. Subsequently, selected cytokines (tumour necrosis factor (TNF), TNF receptors I and II, interleukin (IL) 6, interferon , transforming growth factor (TGF) β and vascular endothelial growth factor (VEGF)), as well as protein carbonylation (oxidative stress index) were determined using an enzyme linked immunosorbent assay (ELISA) in all muscles.

Results: Compared with controls, the vastus lateralis of patients with COPD showed significantly lower protein ELISA levels of TNF, which positively correlated with their quadriceps function, TNF receptor II and VEGF. Protein ELISA levels of IL6, interferon and TGFβ did not differ between patients and controls. Quadriceps protein carbonylation was greater in patients and inversely correlated with quadriceps strength among them.

Conclusions: These findings do not support the presence of a proinflammatory environment within the quadriceps muscles of clinically and weight stable patients with severe COPD, despite evidence for increased oxidative stress and the presence of muscle weakness.

This article has been cited by other articles:

				D. D Sin and W D. Reid Is inflammation good, bad or irrelevant for skeletal muscles in COPD? Thorax, February 1, 2008; 63(2): 95 - 96. [Full Text] [PDF]