Thorax 2000;55:205-209 ( March )

Exhaled 8-isoprostane as a new non-invasive biomarker of oxidative stress in cystic fibrosis

Paolo Montuschi^b, Sergei A Kharitonov^a, Giovanni Ciabattoni^b, Massimo Corradi^a, Liesbeth van Rensen^a, Duncan M Geddes^a, Margaret E Hodson^a, Peter J Barnes^a

^a Department of Thoracic Medicine, Imperial College School of Medicine at the National Heart and Lung Institute, London SW3 6LY, UK, ^b Institute of Pharmacology, School of Medicine, Catholic University of the Sacred Heart, Roma, Italy

Correspondence to: Professor P J Barnes

Received 12 July 1999; Returned to authors 20 September 1999; Revised version received 5 November 1999; Accepted for publication 25 November 1999

BACKGROUNDCystic fibrosis is characterised by oxidative stress in the airways. Isoprostanes are prostaglandin isomers formed by free radical catalysed peroxidation of arachidonic acid. 8-Isoprostane is increased in interstitial lung diseases, asthma, chronic obstructive pulmonary disease, and adult respiratory distress syndrome. Exhaled nitric oxide (NO) and carbon monoxide (CO) are biomarkers of inflammation and oxidative stress in the airways, respectively.
METHODSConcentrations of 8-isoprostane in the breath condensate of 10 normal subjects and 19 patients with stable cystic fibrosis were measured using an enzyme immunoassay (EIA). Breath condensate is a non-invasive method of collecting airway secretions. Exhaled nitric oxide (NO) and carbon monoxide (CO) levels were measured by a chemiluminescence analyser.
RESULTSConcentrations of 8-isoprostane in the breath condensate of patients with stable cystic fibrosis were increased about threefold compared with normal subjects (42.7 (4.5) pg/ml vs 15.2 (1.7) pg/ml; p<0.005, 95% CI 14.6 to 40.9). 8-Isoprostane concentrations were negatively correlated with forced expiratory volume in one second in patients with cystic fibrosis (r = 0.61; p<0.005). Exhaled CO was also increased in patients with cystic fibrosis compared with normal subjects (6.7 (1.2) ppm vs 2.9 (0.3) ppm; p<0.05, 95% CI 0.2 to 7.4). 8-Isoprostane concentrations were significantly correlated with CO levels (r = 0.66; p<0.002).
CONCLUSIONSThe results of this study show that oxidative stress is increased in cystic fibrosis and may be quantified by measuring 8-isoprostane concentrations in breath condensate.


Keywords: cystic fibrosis; 8-isoprostane; oxidative stress

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